Restriction endonuclease cleaving palindromic DNA

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435199, 435824, 536 27, C12P 1934, C12N 922, C12R 1025, C07H 2104

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047466090

ABSTRACT:
The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained from Achromobacter species 718, DSM 2969, is useful in obtaining DNA fragments, e.g., for determining nucleotide sequences and other types of analysis of DNA.

REFERENCES:
Bolton, B. J., Nesch, G., Comer, M. J., Wolf, W. and Kessler, C. (1985) F.E.B.S. Letters 182(1), 130-134.
Roberts, R. J. (1985) Nucleic Acids Research 13 (suppl), r165.

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