Restriction amplification assay

Details Restriction amplification assay Restriction amplification assay

1990-05-04

1992-04-07

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435194, 536 26, 536 27, 536 28, 536 29, 436501, 436 94, C12Q 168, C12N 912, G01N 33566, G01N 3348

Patent

active

051027844

ABSTRACT:
The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. A second oligonucleotide is present in the reaction that is homologous to the 3 prime end of the probe molecule and also conains 5 prime base or bases that will reconstitute the restriction enzyme site on the target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

REFERENCES:
patent: 4725537 (1988-02-01), Fritsch et al.

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