Restriction amplification assay

Details Restriction amplification assay Restriction amplification assay

1993-08-31

1995-09-19

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, 435 912, C12Q 168, C12P 1934

Patent

active

054515025

ABSTRACT:
The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

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Krupp et al., FEBS Letters 212(2):271-275 (Feb. 1987).
"Human Papilloma Virus Type 16 DNA Sequence," Klaus Seedorf, et al., Virology, vol. 145, (1985) pp. 181-185.

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