Respiratory nitrate reductase alpha subunit

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435 691, 435 697, 435183, 4353201, 435191, 4352523, 4352528, C12N 902, C12N 1503, C12N 1553, C12N 1562

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active

061176621

ABSTRACT:
The invention provides respiratory nitrate reductase alpha subunit polypeptides and polynucleotides encoding respiratory nitrate reductase alpha subunit polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing respiratory nitrate reductase alpha subunit polypeptides to screen for antibacterial compounds.

REFERENCES:
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Sun, et al., "Regulators of Aerobic and Anaerobic Respiration in Bacillus subtilis", Journal of Bacteriology, vol. 178, No. 5, pp. 1374-1385, Mar., 1996.
Nakano, et al., "Nitrogen Regulation of nasA and the nasB Operon, Which Encode Genes Required for Nitrate Assimilation in Bacillus subtilis", Journal of Bacteriology, vol. 177, No. 3, pp. 573-579, Feb., 1995.
Pantel, et al., "Staphylococcus carnosus NarG, NarH, NarJ, and Nari genes, complete cds", GenBank Submission, Accession No. AF029225, Nov. 30, 1998.
Bjorn, et al., "Sequence 44 from patent US 5621074", GenBank Submission, Accession No. I40426, Apr. 15, 1997.
Boursier, et al., "Nitrate Reductase Alpha Chain (EC 1.7.99.4).", SwissProt Submission, Accession No. P42175, Nov. 1, 1995.
Burke, et al., "Partial Purification and Some Properties of the Staphylococcus aureus Cytoplasmic Nitrate Reductase", Journal of Bacteriology, vol. 139, No. 1, pp. 120-125, Jul., 1979.
Fang, et al., "Antimicrobial Effect and Disappearance of Sodium Nitrite in Staphylococcus aureus Cultures", Journal Food Science, vol. 50, pp. 1412-1416, (1985).
Hoffman, et al., "The Anaerobic life of Bacillus subtilis: Cloning of the Genes Encoding the Respiratory Nitrate Reductase System", FEMS Microbiology Letters, vol. 131, No. 2, pp. 219-225, (1995).
International Search Report, completed Jul. 29, 1999, from corresponding International Patent Application No. PCT/US99/11319, filed May 21, 1999.

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