Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Patent
1998-10-19
2000-09-05
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
435 691, 435419, 435469, 435470, 800288, 800292, 800293, 800294, 800298, 800320, 8003203, C12N 504, C12N 510, C12N 1582, C12N 1587, A01H 500
Patent
active
061146005
ABSTRACT:
The present invention provides methods for producing a transgenic cell having a stably integrated, single copy of an exogenous polynucleotide sequence. The method, which resolves repeated insertions of the introduced polynucleotide sequence into a single copy, involves introducing into a genetic locus of a cell a polynucleotide sequence flanked on each end by a recombination site. The recombination sites are oriented such that contact with a recombinase would not result in deletion of the polynucleotide sequence from the construct. The multiple, tandem copies of the introduced polynucleotide locus are then contacted with a recombinase that catalyzes recombination among the recombination sites. As a result of this method, the multiple, tandem copies are resolved to a single copy.
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Ow David W.
Srivastava Vibha
Connor Margaret A.
Fado John D.
Mehta Ashwin D.
Silverstein M. Howard
Smith Lynette R. F.
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