Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Reexamination Certificate
1997-07-17
2001-07-17
Robinson, Douglas W. (Department: 1649)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
C536S023200, C536S023600, C536S024100, C536S024300, C435S173300, C435S320100, C435S252300, C435S419000, C435S200000, C435S069100, C800S281000
Reexamination Certificate
active
06262338
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to new resistance genes which have been isolated from plants and to their use for the, transformation of vectors, host organisms and plants, and to the production of plants which exhibit an increased resistance to pests.
2. Description of the Related Art
Many plants are provided with natural possibilities for resisting pests. These resistance mechanisms are controlled by resistance genes. They can be activated by biotic stimuli (for example attack by pests) or abiotic stimuli (for example UV light). In most cases, the mechanisms of these resistances are not yet fully known. There is a demand for having resistance genes available which are primarily activated by pests and which can be incorporated into the genome of plants to further increase the latter's already existing resistance to pests. Such resistance genes which can specifically be induced by pests were hitherto unknown.
SUMMARY OF THE INVENTION
New resistance genes have now been isolated from plants, and these resistance genes can be incorporated into the hereditary matter (the genome) of plants which produce no, or insufficient amounts of, repellent substances, or produce the latter at too late a point in time, it being possible in this manner to bring about increased resistance of these plants to pests (pathogens).
The new resistance genes are characterised in that they can be specifically induced by pathogens and in that they contain DNA sequences which correspond to the cDNA of the following sequence:
1 50
ATCTCGTTCA AGTCGGCGCT CTGGTTGTGG ATGACAGAGC AGAAACCAAA
51 100
ACCTTCTTGC CACAACGTCA TGGTTGGGAA TTACGTGCCA ACAGCATCTG
101 150
ATAGAGCAGC AAATAGAACC TTAGGGTTTG GGTTGGTTAC GAACATCATC
151 170
AACGGCGGCC TGGACTGCGG
It is surprising that a new type of resistance genes could be found which contain sequences which correspond completely, or essentially, to the abovementioned cDNA. This cDNA sequence is novel, and its existence could not have been anticipated.
Furthermore, it must be regarded as surprising that the new resistance genes are essentially activated by pathogens (pests) or by cell-wall fragments of pathogens (elicitors), while other, conventional biotic and abiotic inductors have no substantial effect. The new resistance genes are therefore highly suitable for increasing resistances to pests in transgenic plants.
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Herget Thomas
Schell Jeff
Schreier Peter
Bayer Aktiengesellschaft
Haas Thomas
Robinson Douglas W.
Sprung Kramer Schaefer & Briscoe
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