Reproducible generation of high yields of hepatitis A virus by c

Details Reproducible generation of high yields of hepatitis A virus by c Reproducible generation of high yields of hepatitis A virus by c

1991-09-06

1993-12-07

Ellis, Joan

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 693, 4352351, 435 9133, 536 2372, 935 32, 935 34, 935 57, 935 65, 935 70, C12P 2102, C12P 1934, C12N 700, C12N 500

Patent

active

052682927

ABSTRACT:
The invention is a process for cell propagation of hepatitis A virus. The process involves purifying a culture of hepatitis A virus particles. These hepatitis A virus particles are used for infecting cells being cultivated in a media. The hepatitis A particles suitable for use in this invention must have a cytopathic effect in the infected host cells that are selected for culturing the hepatitis A virus. After developing the cytopathic effect in the infected cells, the hepatitis A viruses produce degeneration and lysis in the infected cells and are released into the media. Isolation of the released hepatitis A virus then occurs. The preferred cells for use in this invention are FRhK4 (foetal rhesus monkey kidney) cells. The preferred virus particles for use in this invention are hepatitis A, strain HAS-15 particles have an antigen coded by a nucleotide sequence according to FIG. 1.

REFERENCES:
patent: 4301249 (1981-11-01), Markus et al.
patent: 4506016 (1985-03-01), Flehmig
patent: 4532215 (1985-07-01), Daemer et al.
patent: 4596674 (1986-06-01), Emini et al.
patent: 4616793 (1986-09-01), Hughes et al.
patent: 4620978 (1986-11-01), Daemer et al.
patent: 4636469 (1987-01-01), Daemer et al.
patent: 4721675 (1988-01-01), Chan et al.
patent: 4861706 (1989-08-01), Hurni et al.
patent: 4894228 (1990-01-01), Purcell et al.
patent: 5021348 (1991-06-01), Giesa et al.
Anderson et al. J. Cell. Biochem. vol. 10D p. 287 (1986).

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