Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-09-09
1999-03-23
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 697, 435 9141, 4353201, 435325, 4352542, 530350, 536 234, 536 235, 536 237, 536 2433, C12Q 168, C12N 1563, C12N 510, C12N 1511
Patent
active
058857796
ABSTRACT:
The invention provides a system for assaying protein-protein interactions. Amino acid sequences of interest are inserted into `bait` and `prey` fusion proteins. The bait fusion proteins include a DNA-binding domain. The prey fusion proteins include a transcriptional repression domain. The interaction of the bait and prey fusion proteins is detected by the repression of reporter genes. The reporter genes have operator sequences to which the DNA-binding domain of the bait fusion protein can bind. The reporter genes are expressed unless the prey fusion protein interacts with a bait fusion protein at the operator region of the reporter gene. When a prey fusion protein binds to a bait fusion protein, the expression of the reporter gene is repressed. Compounds that interfere with the interaction of bait and prey fusion proteins may be detected by their ability to reverse the repression of the reporter genes.
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Hirst Martin
Rohde John
Sadowski Ivan
Brusca John S.
Elliott George C.
Field Bret E.
University of British Columbia
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