Representational fragment amplification

Details Representational fragment amplification Representational fragment amplification

2005-09-07

2008-03-04

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07338765

ABSTRACT:
Methods and kits are provided for non-preferential amplification of a population of nucleic acids. The methods allow for a high degree of amplification, where representation in the final population has a direct linear relationship with the starting material. The amplification product is useful as a probe for hybridization; for generation of libraries, for sequencing, and the like.

REFERENCES:
patent: 6017701 (2000-01-01), Sorge et al.
patent: 6270966 (2001-08-01), Weinstein et al.
patent: 6280948 (2001-08-01), Guilfoyle et al.
patent: 6291170 (2001-09-01), Van Gelder
patent: 2003/0027141 (2003-02-01), MacLeod et al.
patent: 2003/0039992 (2003-02-01), Chakrabarti et al.
Hubank et al., Identifying differences in nRNA expression b y representational difference analysis of cDNA, (1994), N.A.R., 22:5640-5468.
Lisitsyn et al., Cloning the differences between two complex genomes, (1993), Science, 259:946-951.
Ramsay, DNA chips: state-of-the-art, (1998), Nat. Biotech., 16:40-44.
Seth et al., SMART amplification maintains representation of relative gene expression: quantitative validation by real time PCR and application to studies of alcoholic liver disease in primates, (2003), J Biochem Biophys Methods, 55(1):53-66.
Smith et al., Single primer amplification (SPA) of cDNA for microarray expression analysis, (2003), Nucleic Acids Res., 31(3):e9.
Walker et al., Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system (1992), P.N.A.S., 89(1):392-6.

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