Reporter assay screens for protein targets in Saccharomyces...

Details Reporter assay screens for protein targets in Saccharomyces... Reporter assay screens for protein targets in Saccharomyces...

2011-01-18

2011-01-18

Joike, Michele K (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S254200, C435S320100

Reexamination Certificate

active

07872115

ABSTRACT:
The present invention comprises responsive promoters along with screening methods which make use of the promoters in order to identify anti-fungal substances.

REFERENCES:
patent: 5527687 (1996-06-01), Kirsch et al.
patent: 6602699 (2003-08-01), Kozian et al.
patent: 2005/0112573 (2005-05-01), Iwahashi et al.
patent: 2007/0031851 (2007-02-01), Velculescu et al.
GenBank accession No. AX596774.
GenBank accession No. AY189982.
New et al. Reporter Gene Assays and their Applications to Bioassays of Natural Products. Phytother. Res. 17: 439-448, 2003.
Harashima et al. Application of the pho5-gene fusion technology to Molecular Genetics and Biotechnology in Yeast. J. Biosci. & Bioengineer. 91(4): 325-338, 2001.
Fleischmann et al. Allele-specific supression of aSaccharomyces cerevisiaeprp20 mutation by overexpression of a nuclear serine/threonine protein kinase. Mol. Gen. Genet. 250: 614-625, 1996.
Fincham et al. Transformation in Fungi. Micbiol. Review 53(1): 148-170, 1989.
Comino et al. Ecm11 is located in the cell nucleus throughout mitosis. Pflugers Arch.—Eur J Physiol. 442 [Suppl. 1]: R179-R181, 2001.
Accession No. U33007.
Elledge et al. Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase. Genes Dev. 4: 740-751, 1990.
Accession No. AY692668.
http://db.yeastgenome.org/cgi-bin/locus.pl?locus=RNR3.
http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=yil066w.
http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=yil066w, 2009.
Accession No. U33007, 2008.
Accession No. AY692668, 2008.
http://db.yeastgenome.org/cgi-bin/locus.pl?locus=RNR3, 2008.
Accession No. AX596774, 2008.
Accession No. AY189982, 2008.
Alksne, L.E., Identification and Analysis of Bacterial Protein Secretion Inhibitors Utilizing a SecA-LacZ Reporter Fusion System. Antimicrob. Agents Chemother, 44(6)1418-1427 (2000).
Bianchi, Allison A., Stress Responses as a Tool to Detect and Characterize the Mode of Action of Antibacterial Agents. Applied and Environmental Microbiology, 65(11):5023-5027 (1999).
Billinton, N., et al., Development of a Green Fluorescent Protein Reporter for a Yeast Genotoxicity Biosensor. Biosensors & Bioelectronics, 13:831-838 (1998).
Dixon, Graham, et al., A Reporter Gene Assay for Fungal Sterol Biosynthesis Inhibitors. J. Steroid Biochem. Molec. Biol. 62(2-3):165-171 (1997).
Fischer, Hans Peter, et al., Identification of Antibiotic Stress-Inducible Promoters: A Systemic Approach to Novel Pathway-Specific Reporter Assays for Antibacterial Drug Discovery. Genome Res., 14(1):90-98 (2004).
Greer, Lee F., et al., Imaging of Light Emission from the Expression of Luciferases in Living Cells and Organisms: a Review. Luminescence 17:43-74 (2002).
Leskinen, P., et al., One-Step Measurement of Firefly Luciferase Activity in Yeast. Yeast, 20:1109-1113 (2003).
Lewis, Jennifer C., et al., Applications of Reporter Genes. Analytical Chemistry News & Features. 579-585 (1998).
Marathe, Sudhir V., et al., Vectors with the gus reporter gene for identifying and quantitating promoter regions inSaccharomyces cerevisiae. Gene. 154:105-107 (1995).
Shapiro, Elyse, et al., Stress-Based Identification and Classification of Antibacterial Agents: Second-GenerationEscherichia coliReporter Strains and Optimization of Detection. Antimicrobial Agents and Chemotherapy, 46(8):2490-2497 (2002).
Srikantha, Thyagarajan, et al., The Sea PansyRenilla reniformisLuciferase Serves as a Sensitive Bioluminescent Reporter for Differential Gene Expression inCandida albicans. Journal of Bacteriology, 178(1):121-129 (1996).
Szittner, Rose, et al., Bright Stable Luminescent Yeast Using Bacterial Luciferase as a Sensor. Biochemical and Biophysical Research Communications, 309:66-70 (2003).
Vanoni, Marco, et al., Secretion ofEscherichia coliβ-Galactosidase inSaccharomyces cerevisiaeUsing the Signal Sequence from the Glucoamylase-Encoding STA2 Gene. Biochemical and Biophysical Research Communications, 164(3):1331-1338 (1989).
Voth, Warren P., et al., Yeast Vectors for Integration at the HO Locus. Nucleic Acids Research, 29(12 e59):1-4 (2001).
Sun, Dongyu, et al., A Pathway-specific Cell Based Screening System to Detect Bacterial Cell Wall Inhibitors. Journal of Antibiotics, 55(3):279-287 (2002).
SaccharomycesGenome Database record: YLR330W, 2007.
SaccharomycesGenome Database record: YHR012W, 2007.
SaccharomycesGenome Database record: YKL053W, 2007.
SaccharomycesGenome Database record: YKL196C, 2007.
SaccharomycesGenome Database record: YDL103C, 2007.
SaccharomycesGenome Database record: YLR427W, 2007.
SaccharomycesGenome Database record: YLL020C, 2007.
SaccharomycesGenome Database record: YBL065W, 2007.
SaccharomycesGenome Database record: YPL033C, 2007.
SaccharomycesGenome Database record: YOL058W, 2007.
SaccharomycesGenome Database record: YOR255W, 2007.
SaccharomycesGenome Database record: YDR250C, 2007.
SaccharomycesGenome Database record: YDR446W, 2007.
SaccharomycesGenome Database record: YDR536W, 2007.
SaccharomycesGenome Database record: YDR243C, 2007.
SaccharomycesGenome Database record: YDR256C, 2007.
SaccharomycesGenome Database record: YFL020C, 2007.
SaccharomycesGenome Database record: YPL205C, 2007.
SaccharomycesGenome Database record: YGL205W, 2007.
SaccharomycesGenome Database record: YGL117W, 2007.
SaccharomycesGenome Database record: YHR029C, 2007.
SaccharomycesGenome Database record: YML116C, 2007.
SaccharomycesGenome Database record: YJR109C, 2007.
SaccharomycesGenome Database record: YCL030C, 2007.
SaccharomycesGenome Database record: YLR338W, 2007.
SaccharomycesGenome Database record: YIL066W-A, 2007.
SaccharomycesGenome Database record: YNL093W, 2007.
SaccharomycesGenome Database record: YRL381W, 2007.
SaccharomycesGenome Database record: YKL159C, 2007.
SaccharomycesGenome Database record: YIL058W, 2007.
SaccharomycesGenome Database record: YKR037C, 2007.
SaccharomycesGenome Database record: YNL279W, 2007.
SaccharomycesGenome Database record: YOR032C, 2007.
SaccharomycesGenome Database record: YML058W, 2007.
SaccharomycesGenome Database record: YMR303C, 2007.
SaccharomycesGenome Database record: YLR092W, 2007.
SaccharomycesGenome Database record: YFLO52W, 2007.
SaccharomycesGenome Database record: YIR017C, 2007.
SaccharomycesGenome Database record: YLL062C, 2007.
SaccharomycesGenome Database record: YMR323W, 2007.
SaccharomycesGenome Database record: YPL171C, 2007.
SaccharomycesGenome Database record: YER065C, 2007.
SaccharomycesGenome Database record: YDR114C, 2007.
SaccharomycesGenome Database record: YJL153C, 2007.
SaccharomycesGenome Database record: YFR026C, 2007.
SaccharomycesGenome Database record: YMR175W, 2007.
SaccharomycesGenome Database record: YOR387C, 2007.

Affiliated with

Also associated with

Rating

Reporter assay screens for protein targets in Saccharomyces... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reporter assay screens for protein targets in Saccharomyces..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reporter assay screens for protein targets in Saccharomyces... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2638728