Replicative RNA-based amplification/detection systems

Details Replicative RNA-based amplification/detection systems Replicative RNA-based amplification/detection systems

1993-09-27

1996-07-02

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9121, C12Q 168

Patent

active

055321267

ABSTRACT:
This invention relates to the use of functional reporter molecules in the detection and measurement of nucleic acid sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of a transcription step between the production of an appropriate reporter molecule and replication based amplification in order to increase the number of detectable species as an indirect reference to target nucleic acid sequence.

REFERENCES:
patent: 4495280 (1985-01-01), Bujard et al.
patent: 4647529 (1987-03-01), Rodland et al.
patent: 4957858 (1990-09-01), Chu et al.
Fleisher, et al., "Metal Complexes Which Target DNA Sites: Coupling Recognition to Reactivity", Nucl. Acids and Mol. Biol., 2, 65-84 (1988).
Krupp et al., FEBS Letters, v. 212, No. 2, Feb. 1987, pp. 271-275.
Melton et al., Nucleic Acids Research, v. 12, No. 18, 1984, pp. 7035-7056.
Murakawa et al., DNA, v. 7, No. 4, 1988, pp. 287-295.

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