Replication of virulent treponema pallidum in tissue culture

Details Replication of virulent treponema pallidum in tissue culture Replication of virulent treponema pallidum in tissue culture

1982-01-27

1984-08-07

Wiseman, Thomas

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435240, 435243, 435245, 435260, C12N 120, C12N 500, C12N 100, C12N 136, C12N 104

Patent

active

044644701

ABSTRACT:
A method and materials are provided for replication of virulent Treponema pallidum in tissue culture, employing a modified Eagle's minimum essential medium, wherein said in vitro cultivated T. pallidum can be utilized as a source of relatively pure organisms, free of host tissue, for the preparation of a vaccine against syphilis and as a source of organisms for use in specific immunological tests for syphilis.

REFERENCES:
patent: 2255079 (1941-09-01), Morrison et al.
patent: 2513327 (1950-07-01), Ichelson
patent: 2709670 (1955-05-01), Ichelson
patent: 3502546 (1970-03-01), Thompson et al.
patent: 4098646 (1978-07-01), Jones et al.
Netzer: Genetic Engineering News 2(6), Nov.-Dec. 1982.
Norris et al.; Infect. Imm. 22, 689, (1978).
Alderete et al.; Infect. Imm. 26, 1048, (1979).
Norris: Infect. Imm. 36, 437, (1982).
Graves et al., Retention of Motility and Virulence of T. pallidum in vitro: Infection and Immunity, 1975, 12(5), 1116-1120, (USA).
Boak et al., Studies on the Cultivation of T. pallidum, American Journal of Syphilis, Gonorrhea, Veneral Diseases 33, 409-415, (1942).
Fitzgerald, The Future of Tissue Culture Methods for Growth of Treponema pallidum in vitro, Sexually Transmitted Diseases, Apr.-Jun., 1980, pp. 97-99.
Musher et al.--The Role of a Vaccine for Syphilis--ibid Oct.-Dec. 1977, pp. 163-166.
Cox, C. D. and M. K. Barber, 1974, Oxygen Uptake by Treponema pallidum, Infect. Immun. 10:123-127.
Fieldsteel, A. H., F. A. Becker, and J. G. Stout, 1977, Prolonged Survival of Virulent Treponema pallidum, (Nichols Strain) in Cell-Free and Tissue Culture Systems, Infect. Immun. 18:173-182.
Fieldsteel, A. H., D. L. Cox, and R. A. Moeckli, 1981, Cultivation of Virulent Treponema pallidum in Tissue Culture, Infect. Immun. 32:908-915.
Fieldsteel, A. H., J. G. Stout, and F. A. Becker, 1981, Role of Serum in Survival of Treponema pallidum in Tissue Culture, in vitro 17:28-32.
Fitzgerald, T. J., R. C. Johnson, J. A. Sykes, and J. N. Miller, 1977, Interaction of Treponema pallidum (Nichols Strain) with Cultured Mammalian Cells: Effects of Oxygen, Reducing Agents, Serum Supplements, and Different Cell Types, Infect. Immun. 15:444-452.
Kimm, G. E., R. H. Allen, M. J. Morton, and J. F. Morgan, 1962, Studies on the in vitro Survival of Virulent Treponema pallidum, Am. J. Hyg. 75:339-356.
Nelson, R. A. Jr., 1948, Factors Affecting the Survival of Treponema pallidum in vitro, Am. J. Hyg. 48:120-132.
Sandok, P. L., L. M. Jenkin, H. M. Matthews, and M. S. Roberts, 1978, Unsustained Multiplication of Treponema pallidum (Nichols Virulent Strain) in vitro in the Presence of Oxygen, Infec. Immun. 19:421-429.
Turner, T. B. and D. H. Hollander, 1957, Biology of the Treponematoses, W.H.O. Monogr. Ser. No. 35.
Weber, M. M., 1960, Factors Influencing the in vitro Survival of Treponema pallidum, Am. J. Hyg. 71:401-417.

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