Repeat insult microbial test method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 4, 435 29, 435299, 435301, 435805, 424407, 424413, 424419, C12Q 102, C12Q 104, C12N 1104, C12N 1100

Patent

active

053506790

ABSTRACT:
A repeat insult microbial test method in which an antimicrobial agent is applied to a porous permeable substrate. The porous permeable substrate is inoculated with microorganisms, and the porous permeable substrate is incubated for a predetermined period of time, at a temperature which is conducive to the flourishment of the microorganisms. The porous permeable substrate is reinoculated and reincubated a predetermined plurality of times and the inoculated porous permeable substrate is incubated for a final period of time between 18-24 hours at the temperature used previously. The porous permeable substrate is then examined, and the growth of microorganisms is determined.

REFERENCES:
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patent: 5206028 (1993-04-01), Li
patent: 5234688 (1993-08-01), Gaffer
Lennette et al., (Ed.), Manual of Clinical Microbiology, Third Edition, Amer. Soc. Microbiology, Washington, D.C., 1980, pp. 463-474.

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