Repair-mediated process for amplifying and detecting nucleic aci

Details Repair-mediated process for amplifying and detecting nucleic aci Repair-mediated process for amplifying and detecting nucleic aci

1993-10-27

1999-12-21

Arthur, Lisa B.

Semiconductor device manufacturing: process

Including control responsive to sensed condition

Interconnecting plural devices on semiconductor substrate

435 912, 435 9152, C12Q 168, C12P 1934

Patent

active

060048263

ABSTRACT:
This invention relates to a process for amplifying and detecting any desired specific nucleic acid sequence that exists in a nucleic acid or mixture thereof. The process comprises treating single strand RNA or separated complementary strands of DNA target with a molar excess of oligonucleotide complement pairs in which these oligonucleotide complement pairs have sequences complementary to the target, under hybridizing conditions. In one embodiment, the oligonucleotide complement pairs may have a gap of one or more bases which may be repaired (filled) by enzymes. The oligonucleotide complement pairs are joined together, forming joined, oligonucleotide product. The target/joined product hybrid nucleic acids are then denatured to single strands again, at which point both the target and the joined products can form hybrids with new oligonucleotide complement pairs. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

REFERENCES:
patent: 4800159 (1989-01-01), Mullis et al.
Takahashi et al., Journal of Biological Chemistry 259 (16) 10,041-10,047 (1984).

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