Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1991-06-26
1992-05-19
Doll, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530412, 530413, 530416, 530417, 5303881, 5303871, 5303895, 435 733, C07K 322, C07K 1528, C07K 1500
Patent
active
051151014
ABSTRACT:
Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.
REFERENCES:
patent: 4833085 (1989-05-01), Schaumann et al.
Martin, L. N. 1982 J. of Immunol Methods 50:319-329.
Manil, et al 1986 J. of Immunol Methods 90:25-37.
Dertzbaugh, et al. J. Of Immunol Methods 83:169-177 1985.
Buckland, 1986 Nature 370:557-558.
Bloom James W.
Mitra Gautam
Wong Melvin F.
Adams Donald E.
Doll John
Giblin James A.
Miles Inc.
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