Removal of phthalyl groups from amides and esters using an amida

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

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435121, 435128, 435142, 435227, 435228, C12N 112, C12N 120, C12N 980

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active

055321496

ABSTRACT:
Phthalyl amidase is an enzyme previously unknown in the art that catalyzes removal of the phthalyl moiety from phthalyl-containing amides. The current invention provides a phthalyl amidase, a method for producing it by culturing the natural organism from which the activity was identified, and methods for using the phthalyl amidase to remove the phthalyl moiety from phthalyl-containing amides. The enzyme is isolated from Xanthobacter agilis.

REFERENCES:
Yang et al., J. Biol. Chem. 268:10870, 1993.
Kukolja et al., Croatica Chemica Acta 49:779, 1977.
Toyoura et al., Chem. Pharm. Bull. 7:789, 1959.

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