Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1991-04-12
1992-10-20
Cashion, Jr., Merrell C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
514 2, 514 12, 514 21, 5303871, 5303881, 530416, 530417, 530418, 530419, 530420, 530421, 530863, 530864, 424 858, C07K 312, C07K 328, C07K 1528
Patent
active
051571137
ABSTRACT:
An essentially pure and stablized antibody preparation comprising IgM antibodies having a purity greater than about 98% by weight and a nucleic acid content of less than about 200 pg per mg IgM. In one embodiment IgM antibodies from a monoclonal source are subjected to ion exchange and size exclusion chromatography at an alkaline pH to yield a purified IgM having a nucleic acid content of less than 10 pg/mg IgM, preferably less than about 4 pg/mg IgM. A highly purified and stabilized preparation of anti Pseudomonas aeruginosa antibodies is disclosed. The removal of nucleic acids is assured by subjecting the antibody source to at least one and preferably two low pH precipitation steps. In a very preferred embodiment, ion exchange and/or size exclusion chromatography is used to remove any residual nucleic acids.
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Dove George
Mitra Gautam
Cashion Jr. Merrell C.
Enayati Elizabeth F.
Giblin James A.
Kozycki Andrew G.
Miles Inc.
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