Removal of nucleic acids from monoclonal antibody preparations

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

514 2, 514 12, 514 21, 5303871, 5303881, 530416, 530417, 530418, 530419, 530420, 530421, 530863, 530864, 424 858, C07K 312, C07K 328, C07K 1528

Patent

active

051571137

ABSTRACT:
An essentially pure and stablized antibody preparation comprising IgM antibodies having a purity greater than about 98% by weight and a nucleic acid content of less than about 200 pg per mg IgM. In one embodiment IgM antibodies from a monoclonal source are subjected to ion exchange and size exclusion chromatography at an alkaline pH to yield a purified IgM having a nucleic acid content of less than 10 pg/mg IgM, preferably less than about 4 pg/mg IgM. A highly purified and stabilized preparation of anti Pseudomonas aeruginosa antibodies is disclosed. The removal of nucleic acids is assured by subjecting the antibody source to at least one and preferably two low pH precipitation steps. In a very preferred embodiment, ion exchange and/or size exclusion chromatography is used to remove any residual nucleic acids.

REFERENCES:
patent: 4172124 (1979-10-01), Koprowski et al.
patent: 4271145 (1981-06-01), Wands et al.
patent: 4272521 (1981-06-01), Zuffi
patent: 4318902 (1982-03-01), Stephan
patent: 4464465 (1984-08-01), Lostrom
patent: 4604235 (1986-08-01), Flashner
WPI Abstract of JP 53-20494 Feb. 24 (1978).
WPI Abstract, Mitsubishi Gas Chem Ind., JP 53-020493 Feb. 24, 1978.
Dove et al., Abstr. Pap. Am. Chem. Soc. 198th Meeting MBTD 26, 1989.
Newkirk et al., Hybridoma, vol. 6, No. 5 pp. 453-460 (1987).
Scopes, Protein Purification--Principles and Practice, Second Edition (Springer-Verlag New York, Inc.) pp. 62-63 (1987).
Clezardin et al.; J. Chromatog., vol. 319 pp. 67-77 (1985).
Sugg et al., "On the Problems of Efficacy of Separation of IgM and IgG by Different Methods", 36 Vox Sang. 25-28 (1979).
Wichman et al., "Purification of Human Immunoglobulin M by Affinity Chromatography on Protamine-Sepharose", 490 Biochem. Biophys. Acta 363-369 (1977).
Sampson et al., "The Separation of Immunoglobulin M from Human Serum by Fast Protein Liquid Chromatography", 69 J. Immuno. Meth., 9-15 (1984).
Kohler and Milstein, "Continuous Cultures of Fused Cells Secreti Antibody of Predefined Specificity", 256 Nature 495-497 (1975).
Steinbuch et al., "Preparation of an IgM and IgA Enriched Fraction for Clinical Use", 3(4) Preparative Biochemistry 363-373 (1973).
Burchiel et al., "Rapid and Efficient Purification of Mouse Monoclonal Antibodies from Ascites Fluid Using High Performance Liquid Chromatography", 69 J. Immuno. Meth., p. 33 (1984).
Deschamps et al., "A High-Performance Liquid Chromatographic Procedure for the Purification of Mouse Monoclonal Antibodies", 147 Anal. Biochem. p. 451 (1985).
Brooks et al., "Preparative HPLC Purification of IgG and IgM Monoclonal Antibodies", Amer. Lab. (Oct. 1985).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Removal of nucleic acids from monoclonal antibody preparations does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Removal of nucleic acids from monoclonal antibody preparations, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Removal of nucleic acids from monoclonal antibody preparations will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-192970

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.