Removal of molecular assay interferences

Details Removal of molecular assay interferences Removal of molecular assay interferences

2001-08-16

2009-08-04

Johannsen, Diana B (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S091500, C435S091520

Reexamination Certificate

active

07569342

ABSTRACT:
Methods and systems for removing masking agents from test samples, e.g., DNA-containing samples obtained from living subjects, when they are submitted for or subjected to molecular assays. The present invention allows molecular assays of nucleic acids in bodily fluids and excretions, such as urine, blood, blood serum, amniotic fluid, spinal fluid, conjunctival fluid, salivary fluid, vaginal fluid, stool, seminal fluid, and sweat to be carried out with greater sensitivity. The masking agents are suppressed by contacting a test sample with an amount of one or more divalent metal chelators and an amount of one or more chelator enhancing components. The amounts of the divalent metal chelator(s) and the chelator enhancing component(s) are selected such that interference of a masking agent on a molecular assay of a nucleic acid-containing test sample are suppressed, and upon contact with the divalent metal chelator(s)/chelator enhancing component(s), the masking agents are suppressed.

REFERENCES:
patent: 3847738 (1974-11-01), Brake et al.
patent: 4040785 (1977-08-01), Kim et al.
patent: 4703008 (1987-10-01), Lin
patent: 4741446 (1988-05-01), Miller
patent: 4812310 (1989-03-01), Sato et al.
patent: 4882270 (1989-11-01), Moroz
patent: 4935342 (1990-06-01), Seligson et al.
patent: 4983523 (1991-01-01), Li et al.
patent: 4991104 (1991-02-01), Miller
patent: 5010183 (1991-04-01), Macfarlane
patent: 5030720 (1991-07-01), Bertland et al.
patent: 5128247 (1992-07-01), Koller
patent: 5149653 (1992-09-01), Roser
patent: 5155018 (1992-10-01), Gillespie et al.
patent: 5192553 (1993-03-01), Boyse et al.
patent: 5217866 (1993-06-01), Summerton et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5300424 (1994-04-01), Hoss et al.
patent: 5300635 (1994-04-01), Macfarlane
patent: 5312744 (1994-05-01), Shibata
patent: 5341692 (1994-08-01), Sher et al.
patent: 5346999 (1994-09-01), Cathcart et al.
patent: 5395498 (1995-03-01), Gombinsky et al.
patent: 5431952 (1995-07-01), Ocello
patent: 5457025 (1995-10-01), Collins et al.
patent: 5459073 (1995-10-01), Ryan
patent: 5459253 (1995-10-01), Wolin et al.
patent: 5464744 (1995-11-01), Farrell et al.
patent: 5501963 (1996-03-01), Burckhardt
patent: 5514551 (1996-05-01), Yang et al.
patent: 5538870 (1996-07-01), Noeth et al.
patent: 5552325 (1996-09-01), Nochumson et al.
patent: 5554503 (1996-09-01), Down et al.
patent: 5595896 (1997-01-01), Coruzzi et al.
patent: 5609864 (1997-03-01), Shanbrom
patent: 5610287 (1997-03-01), Nikiforov et al.
patent: 5614391 (1997-03-01), Franciskovich et al.
patent: 5620852 (1997-04-01), Lin et al.
patent: 5629147 (1997-05-01), Asgari et al.
patent: 5637687 (1997-06-01), Wiggins
patent: 5643767 (1997-07-01), Fischetti et al.
patent: 5654179 (1997-08-01), Lin
patent: 5667963 (1997-09-01), Smith et al.
patent: 5702896 (1997-12-01), Collins et al.
patent: 5716785 (1998-02-01), Van Gelder et al.
patent: 5728822 (1998-03-01), Macfarlane
patent: 5744302 (1998-04-01), Sessler et al.
patent: 5744520 (1998-04-01), Kmiecik-Lawrynowicz et al.
patent: 5747663 (1998-05-01), Colpan et al.
patent: 5753433 (1998-05-01), Kessler et al.
patent: 5759777 (1998-06-01), Kearney et al.
patent: 5763185 (1998-06-01), Collis et al.
patent: 5766843 (1998-06-01), Asgari et al.
patent: 5777098 (1998-07-01), Gray et al.
patent: 5777099 (1998-07-01), Mehra
patent: 5783686 (1998-07-01), Gonzalez
patent: 5784162 (1998-07-01), Cabib et al.
patent: 5786208 (1998-07-01), Clark et al.
patent: 5792651 (1998-08-01), Colpan et al.
patent: 5804684 (1998-09-01), Su
patent: 5808041 (1998-09-01), Padhye et al.
patent: 5811099 (1998-09-01), Ryan
patent: 5817798 (1998-10-01), Gundling
patent: 5830664 (1998-11-01), Rosemeyer et al.
patent: 5834303 (1998-11-01), Fujishiro
patent: 5837466 (1998-11-01), Lane et al.
patent: 5846783 (1998-12-01), Wu et al.
patent: 5849517 (1998-12-01), Ryan
patent: 5857462 (1999-01-01), Thomas et al.
patent: 5858649 (1999-01-01), Asgari et al.
patent: 5860937 (1999-01-01), Cohen
patent: 5861253 (1999-01-01), Asgari et al.
patent: 5871928 (1999-02-01), Fodor et al.
patent: 5879875 (1999-03-01), Wiggins et al.
patent: 5891636 (1999-04-01), Van Gelder et al.
patent: 5906744 (1999-05-01), Carroll et al.
patent: 5910246 (1999-06-01), Walter et al.
patent: 5916775 (1999-06-01), Hayashizaki
patent: 5932422 (1999-08-01), Shyjan et al.
patent: 5939259 (1999-08-01), Harvey et al.
patent: 5945515 (1999-08-01), Chomczynski
patent: 5968746 (1999-10-01), Schneider
patent: 5972222 (1999-10-01), Gjerde et al.
patent: 5972613 (1999-10-01), Somack et al.
patent: 5973137 (1999-10-01), Heath
patent: 5985556 (1999-11-01), Kambara et al.
patent: 5985572 (1999-11-01), Macfarlane
patent: 5990301 (1999-11-01), Colpan et al.
patent: 5990302 (1999-11-01), Kuroita et al.
patent: 6001610 (1999-12-01), Seibl et al.
patent: 6004762 (1999-12-01), Tse et al.
patent: 6020186 (2000-02-01), Henco et al.
patent: 6027750 (2000-02-01), Gautsch et al.
patent: 6027890 (2000-02-01), Ness et al.
patent: 6030527 (2000-02-01), Gjerde et al.
patent: 6030608 (2000-02-01), Hoyes et al.
patent: 6032474 (2000-03-01), Dale et al.
patent: 6037465 (2000-03-01), Hillebrand et al.
patent: 6043032 (2000-03-01), Yamagishi
patent: 6043354 (2000-03-01), Hillebrand et al.
patent: 6066455 (2000-05-01), Kruse-Mueller et al.
patent: 6071395 (2000-06-01), Lange
patent: 6071745 (2000-06-01), Lin et al.
patent: 6084091 (2000-07-01), Müller et al.
patent: 6090936 (2000-07-01), Walter et al.
patent: 6110676 (2000-08-01), Coull et al.
patent: 6111096 (2000-08-01), Laugharn, Jr. et al.
patent: 6114108 (2000-09-01), Budowsky
patent: 6117398 (2000-09-01), Bienhaus et al.
patent: 6120985 (2000-09-01), Laugharn, Jr. et al.
patent: 6121055 (2000-09-01), Hargreaves
patent: 6133036 (2000-10-01), Putcha et al.
patent: 6136555 (2000-10-01), Jones
patent: 6156206 (2000-12-01), Gjerde et al.
patent: 6165773 (2000-12-01), New et al.
patent: 6168922 (2001-01-01), Harvey et al.
patent: 6177278 (2001-01-01), Haj-Ahmad
patent: 6197506 (2001-03-01), Fodor et al.
patent: 6203993 (2001-03-01), Shuber et al.
patent: 6210881 (2001-04-01), Little et al.
patent: 6218531 (2001-04-01), Ekenberg
patent: 6225052 (2001-05-01), Batz et al.
patent: 6231815 (2001-05-01), Bainczyk et al.
patent: 6232464 (2001-05-01), Lange
patent: 6235501 (2001-05-01), Gautsch et al.
patent: 6248522 (2001-06-01), Haberhausen et al.
patent: 6248585 (2001-06-01), Berd
patent: 6248588 (2001-06-01), Crespo et al.
patent: 6251638 (2001-06-01), Umansky et al.
patent: 6251660 (2001-06-01), Muir et al.
patent: 6258320 (2001-07-01), Persing et al.
patent: 6258930 (2001-07-01), Gauch et al.
patent: 6268136 (2001-07-01), Shuber et al.
patent: 6274308 (2001-08-01), Lee et al.
patent: 6274386 (2001-08-01), Harttig
patent: 6280724 (2001-08-01), Moore
patent: 6281002 (2001-08-01), Möller-Bremer
patent: 6281349 (2001-08-01), Pulleyblank
patent: 6287820 (2001-09-01), Umansky et al.
patent: 6291178 (2001-09-01), Schneider
patent: 6291248 (2001-09-01), Haj-Ahmad
patent: 6303315 (2001-10-01), Skouv
patent: 6306345 (2001-10-01), Bronshtein et al.
patent: 6322981 (2001-11-01), Rodgers et al.
patent: 6331527 (2001-12-01), Parmacek et al.
patent: 6342387 (2002-01-01), Hayashizaki et al.
patent: 6348336 (2002-02-01), Matveld et al.
patent: 6352838 (2002-03-01), Krulevitch et al.
patent: 6355792 (2002-03-01), Michelsen et al.
patent: 6391541 (2002-05-01), Petersen et al.
patent: 6409528 (2002-06-01), Bodnar
patent: 6410321 (2002-06-01), Lin et al.
patent: 6458546 (2002-10-01), Baker
patent: 6465209 (2002-10-01), Blinkovsky et al.
patent: 6465639 (2002-10-01), van Gemen et al.
patent: 6468782 (2002-10-01), Tunnacliffe et al.
patent: 6471866 (2002-10-01), Gjerde et al.
patent: 6478967 (2002-11-01), Mü ller
patent: 6492162 (2002-12-01), Sakurai et al.
patent: 6509146 (2003-01-01), Bronshtein
patent: 6514943 (2003-02-01), Kovesdi et al.
patent: 6528641 (2003-03-01), Lader
patent: 6537745 (2003-03-01), Chien et al.
patent: 6541204 (2003-04-01), Nilsen et al.
patent: 6545144 (2003-04-01), Kolzau et al.
patent: 6548256 (2003-04-01), Lienau et al.
patent: 6551777 (2003-04-0

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