Compositions – Miscellaneous
Patent
1979-06-25
1981-02-10
Schain, Howard E.
Compositions
Miscellaneous
23230B, 210927, 260112B, 260121, 424 2, 424 9, 424101, 536 21, A61K 3516, A61K 4900, C08B 3710, G01N 100
Patent
active
042500419
ABSTRACT:
This invention relates to a method for the removal of heparin from heparin-containing plasma test samples using an insoluble protamine reaction product, without adversely affecting subsequent testing of the plasma for clotting time. Protamine sulfate or a combination of protamine sulfate and serum albumin is cross-linked with glutaraldehyde to form an insoluble reaction product which is capable of adsorbing heparin. According to the method of this invention, excess amounts of either of the cross-linked protamine reaction products are added to blood plasma samples containing heparin and the mixture is agitated for a time sufficient to permit adsorption of substantially all heparin present. The insoluble protamine heparin complex formed is removed from the plasma along with any excess insoluble protamine reaction product. Aliquots of the heparin-free plasma may be subjected to coagulation tests in order to determine true clotting time.
REFERENCES:
patent: 4175182 (1979-11-01), Schmer
J. Lab. Clin. Med. 34, pp. 473-476 (1949), Allen et al.
Arch. Biochem. Biophys. 126:10-26 (1968), Habeeb et al.
Arch. Biochem. Biophys. 144:394-400 (1971), Jansen et al.
J. Lab. Clin. Med. 48, 223-226 (1956), Perkins.
Babson Arthur L.
Turner James E.
Miller Stephen I.
Patton Walter
Raines Stephen
Schain Howard E.
Warner-Lambert & Company
LandOfFree
Removal of heparin from blood plasma samples using an insoluble does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Removal of heparin from blood plasma samples using an insoluble , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Removal of heparin from blood plasma samples using an insoluble will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1664493