Removal of bacterial endotoxin in a protein solution by...

Details Removal of bacterial endotoxin in a protein solution by... Removal of bacterial endotoxin in a protein solution by...

2005-03-23

2010-11-30

Therkorn, Ernest G (Department: 1797)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C530S413000, C536S023100, C536S023700

Reexamination Certificate

active

07842187

ABSTRACT:
The present invention relates to the purification of polypeptides and the removal of endotoxin via immobilized metal affinity chromatography (IMAC). More specifically, the invention relates to methods for removing bacterial endotoxin in a protein solution. In specific embodiments, the invention relates to the elimination of endotoxin fromMoraxella catarrhalisouter membrane proteins.

REFERENCES:
patent: 3897309 (1975-07-01), Grabner
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patent: 4381239 (1983-04-01), Chibata et al.
patent: 6310190 (2001-10-01), Hansen et al.
Duff et al., Clinical Research, 30, 565A, 1982.
Issekutz, Journal of Immunological Methods, 61:275-281, 1983.
Morrison et al., Immunochemistry, 13:813-818, 1976.

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