Release and isolation of N-glycans and O-glycans

Details Release and isolation of N-glycans and O-glycans Release and isolation of N-glycans and O-glycans

1993-03-12

1996-07-23

Robinson, Douglas W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 187, 536 221, 536 552, 536 553, 536124, C07H 504, C07H 506, C07H 100, C08B 3700

Patent

active

055390900

ABSTRACT:
N- and O-glycans are released from a glycoconjugate with a substantially anhydrous hydrazine reagent, the glycoconjugate being substantially salt-free and substantially anhydrous. Released N- and O-glycans are recovered in substantially unreduced and intact form using a chromatographic material.

REFERENCES:
patent: 4719294 (1988-01-01), Rademacher et al.
patent: 4736022 (1988-04-01), Rademacher et al.
J. Montreuil et al., "Carbohydrate Analysis--A Practical Approach," IRL Press 1986.
CARBOHYDRATE RESEARCH, vol. 151, 1986, pp. 89-103, B. Bendiak et al "Purification of Oligosaccharides Having a Free Reducing-end from Glycopeptide Sources".

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