Regulatory system for expression of nitrilase gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound

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435 691, 4352523, 4353201, 935 22, 935 23, 935 27, 935 33, 935 39, 935 72, 536 237, C12N 1500, C12N 1509, C12N 121, C12P 2100

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056020147

ABSTRACT:
The invention relates to a regulatory factor or system composed of 2 components of a polypeptide having the amino acid sequence of SEQ ID No:1 and a polypeptide having the amino acid sequence of SEQ ID No:2 to activate a nitrilase gene promoter, as well as to DNA coding therefor. Nitrilase can be produced by introducing the gene coding for the present regulatory factor together with a nitrilase gene containing a promoter region into a microorganism of the genus Rhodococcus.

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