Regulatory sequences and expression cassettes for yeasts,...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S024100, C435S320100, C435S069100, C435S071100, C435S252300

Reexamination Certificate

active

07132522

ABSTRACT:
The invention relates to a DNA sequence which acts as a promoter in yeast cells, to an expression and optionally, secretion system containing said DNA sequence, to plasmids containing this system, to host cells that have been transformed with said DNA and to methods for producing proteins and polypeptides.

REFERENCES:
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patent: 4943529 (1990-07-01), Van den Berg et al.
Siekstele et al. Cloning, Targeted Disruption and Heterologous Expression of theKluyveromyces marxianusEndopolygalcturonase Gene (EPG1) Yeast vol. 15 No. 4 Mar. 15, 1999 pp. 311-322.
Accession No. AF000076, Mar. 17, 1999.
Bartkeviciute et al. Construction of Stable Autonomously Replicating Vector for YeastKluyveromyces. Biologija2:22-23 (1996).
Bird et al. The tomato polygalacturonase gene and ripening-specific expression in transgenic plants.Plant Molecular Biology II11:651-662 (1998).
Bussink et al. Characterization of polygalacturonase-overproducingAspergilus nigertransformants.Appl. Microbiol. Biotechnol. 37:324-329 (1992).
Nicholass et al. High levels of ripening-specific reporter gene expression directed by tomato fruit polygalacturonase gene-flanking regions.Plant Molecular Biology28:423-435 (1995).
Siekstele et al. Cloning, Targeted Disruption and Heterologous Expression of theKluyveromyces marxianusEndopolygalacturonase Gene (EPG1).Yeast15:311-322 (1999).

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