Regulatory sequence

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C435S415000, C435S416000, C435S417000, C435S419000, C435S468000, C536S024100, C800S295000

Reexamination Certificate

active

10541315

ABSTRACT:
The present invention relates to the field of plant molecular biology. In particular, it describes the use of a regulatory nucleic acid sequence of the rice gene GOS2 for the regulation of gene expression in plant cells derived from plants other than monocotyledonous plants. The use of the regulatory sequence of the present invention results in constitutive expression with expression levels similar to that of CaMV 35S. The present invention also relates to vectors and host cells comprising these nucleic acid sequences. The invention further relates to transgenic cells and plants comprising these sequences and to methods for obtaining such cells and plants.

REFERENCES:
patent: 6066456 (2000-05-01), Bridges et al.
De Pater et al The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1. The Plant Journal 1992 2:6 837.
International Search Report for PCT/EP2004/000645 dated Aug. 19, 2004.
Pater et al., The Promoter of the Rice Gene GOS2 Is Active In Various Different Monocot Tissues and Binds Rice Nuclear Factor ASF-1,Plant Journal, vol. 2, No. 6, 1992, pp. 837-844, XP000907326.
Quellet et al., The wheat wcs120 promoter is cold-inducible in both monocotyledonous and dicotyledonous species,FEBS Letters, vol. 423, No. 3, Feb. 27, 1998, pp. 324-328, XP004261912.
Luan et al., A Rice Cab Gene Promoter Contains Separate CIS-acting Elements that Regulate Expression in Dicot and Monocot Plants,Plant Cell, vol. 4, No. 8, 1992, pp. 971-981, XP002283900.
Hauptmann et al., Promoter Strength Comparisons of Maize Shrunken 1 and Alcohol Dehydrogenase 1 and 2 Promoters in Monocotyledonous and Dicotyledonous Species,Plant Physiology, vol. 88, No. 4, 1988, pp. 1063-1066, XP001182033.
Database Biosis, Deng et al.,Expression of a zein gene Z4 introduced into dicotyledonous Solanum nigrum, XP002283901.

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