Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-09-19
2006-09-19
Achutamurthy, P. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S004000, C435S006120, C435S252300, C435S320100, C435S440000, C435S069100, C435S071100, C536S023100, C536S023200, C536S023700
Reexamination Certificate
active
07109009
ABSTRACT:
Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex ofC. tropicalis20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
REFERENCES:
patent: 5254466 (1993-10-01), Picataggio et al.
patent: 5620878 (1997-04-01), Picataggio et al.
patent: 5648247 (1997-07-01), Picataggio et al.
patent: 6331420 (2001-12-01), Wilson et al.
patent: WO 91/147781 (1991-10-01), None
Sutter et al., NADPH-Cytochrome P450 reductase (EC 1.6.2.4) (CPR), Swissport Sequence Data Base, Oct. 1, 1994, Ac P37201.
Sutter et al., C. tropicalis NADPH-cytochrome P450 reductase gene, complete cds. EMBL Sequence Database, Jul. 21, 1990, Heidelberg DE Ac M35199.
Nelson, D. R. 1996. P450 Superfamily; Update on New sequences, gene mapping, accession numbers, and nomenclature. Pharmacogenetics. 6(1):1-42.
Garfinkel, D. 1958. Studies on pig liver microsomes. I. Enzymatic and pigment composition of different microsomal fractions. Arch. Biochem. Biophys. 77:493-509.
Klaassen, C. D., M. O. Amdur, and J. Doull. 1986. Toxicology, 3rd ed. Macmillan, New York.
Omura, T., and R. Sato. 1964. The carbon-monoxide-binding pigment of liver microsomes. I. Evidence of its hemoprotein nature. J. Biol. Chem. 239:2370-2378.
Goeptar, A. R., Heleen Scheerens and Nico P.E. Vermeulen. 1995. Oxygen and Xenobiotic Reductase Activities of Cytochrome P450. Critical Reviews in Toxicology. 25(1):25-65.
Taniguchi, H., Y. Imai. and R. Sato. 1984. Role of electron transfer system in microsomal drug monooxygenase reaction catalyzed by cytochrome P450. Arch. Biochem. Biophys. 232:585-596.
Potter, D. W., and D. J. Reed. 1983. Involvement of FMN and phenobarbital cytochrome P450 in stimulating a one-electron reductive denitrosation of 1-(2-chloroethyl)-3-(cyclohexyl)-1-nitrosourea catalyzed by NADPH-cytochrome P450 reductase J. Biol. Chem. 258:6906-6911.
Vermilion, J. L., and M. J. Coon. 1978. Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P450 reductase. J. Biol. Chem. 253:8812-8819.
Guengerich, F. P., and M. V. Martin. 1980. Purification of cytochrome P-450, NADPH-cytochrome P-450 reductase and epoxide hydratase from a single preparation of rat liver microsomes. Arch. Biochem. Biophys. 205:365-379.
Ortiz de Montellano, P. R 1986. Cytochrome P450; Structure, Mechanism and Biochemistry. Plenum Press, New York (Table of Contents).
Kuthen, H., and V. Ulrich. 1982. Oxidase and oxygenase function of the microsomal cytochrome P450 monooxygenase system. Eur. J. Biochem. 126:583-588.
Poulos, T. L., and R. Raag. 1992. Cytochrome P450 crystallography, oxygen activation and electron transfer. FASEB J. 6:674-679.
Mukhopadhyay, C. K. a. I. B. C. 1994. NADPH initiated cytochrome P450-mediated free metal ion independent oxidative damage of microsomal proteins. Journal of Biological Chemistry. 269(18):13390-13397.
Ross, A. D., Varghese. G., Oporto. B., Carmichael, F.J., and Isreal Y. 1995. Effects of propylthiouracil treatment on NADPH-cytochrome P450 reductase levels. oxygen consumption and hydroxyl radical formation in liver microsomes from rats fed ethanol or acetone chronically. Biochemical Pharmacology. 49(7):979-989.
Yamazaki, S., Nakano, N., Imai, Y., Ueng, Y.F., Guengerich, F.P., and T. Shimada. 1996. Roles of cytochrome b5 in the oxidation of testosterone and nifedipine by recombinant cytochrome P450 3A4 and by human liver microsomes. Archives of Biochemistry and Biophysics. 325(2):174-182.
Gotoh, O., Tagashira, Y., Iizuka, T., and Y. Fuji-kuriyama. 1983. Structural characteristics of Cytochrome P450. Possible location of the heme-binding cysteine in determined amino acid sequences. J. Biochem. 93(807-817).
Morohashi, K., Sogawa,K., Omura, T., and Y. Fuji-kuriyama. 1987. Gene structure of human cytochrome P450 (SCC), cholestrol desmolase. J. Biochem. 101:879-887.
Kalb, V. and. J. Loper. 1988. Proteins from eight eukaryotic cytochrome P-450 families share a segmented region of sequence similarity. PNAS. 85:7221-7225.
Kaiser, C., S. Michaelis, and A. Mitchell, 1994. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, USA (Title page).
Sambrook, J., E. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press, USA (Title page).
Boeke, J. D., LaCroute. F., and G.R. Fink. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast:5-fluro-orotic acid resistance. Mol. Gen. Genet. (1984) 197:345-346.
Picataggio, S., Deanda, K., and J. Mielenz. Determination ofCandida tropicalisAcyl Coenzyme A Oxidase Isozyme Function by Sequential Gene Disruption. 1991. Mol. and Cell. Biol. 11:4333-4339.
Rohrer, T.L. and S.K. Picataggio. Targeted integrative transformation ofCandida tropicalisby electroporation. Appl. Microbiol. Biotechnol. 1992. 36:650-654.
Picataggio, S., Rohrer, T., Deanda, K., Lanning, D., Reynolds, R., Mielenz, J. and L.D. Eirich. Metabolic engineering ofCandida tropicalisfor the production of long-chain dicarboxylic acids. 1992. Bio/Technology 10:894-898.
Sutter, T.R., Sangard, D. and J.C. Loper. 1990. Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene fromCandida tropicalis. J. Biol. Chem. 265:16428-16436.
Kargel, E., Menzel, R., Honeck, H., Vogel, F., Bohmer, A. and W. Schhunck. 1996.Candida maltosaNADPH-cytochrome P450 reductase: cloning of a full-length cDNA, heterologous expression inSaccharomyces cerevisiaeand function of the N-terminus region of membrane anchoring and proliferation of the endiplasmic reticulum. Yeast. 12:333-348.
Ohkuma, M., Muraoka, S., Tanimoto, T., Fuji. M., Ohta. A. and Takagi, M. 1995. CYP52 (cytochrome P450alk) multigene family inCandida maltosa: identification and characterization of eight members. DNA and Cell Biology. 14:163-173.
Seghezzi, W., Meili. C., Ruffiner. R., Kuenzi. R., Sanglard. D. and A. Fiechter. 1992. Identification and characterization of additional members of the cytochrome P450 multigene family CYP52 ofCandida tropicalis. DNA and Cell Biology. 11.767-780.
Sutter et al., Isolation and Characterization of the Alkane-inducible NADPH-Cytochrome P-450 Oxidoreductase Gene forCandida tropicalis, The Journal of Biological Chemistry, vol. 265, Sep. 25, 1990, pp. 16428-16436.
Ohkuma et al., C. Maltosa ALK2-A and ALK3-A Genes for n-alkane inducible cytochrome P-450, EMBL Sequence Database Mar. 14, 1995, Heidelberg DE Ac X55881.
Ohkuma et al., Cytochrome P450 52D1, Swissprot Sequence Data Base, Dec. 15, 1998, Ac Q12585.
Ohkuma, et al., CYP52 (Cytochrome P450alk) Multigene Family inCandida maltosa: Identification and Characterization of Eight Members, DNA and Cell Biology, Vol. 14, 1995, pp. 163-175.
Kobayashi et al., Quantiative Analysis of Human Multidrug Resistance 1 (MDR1) Gene Expression by Nonisotopic Competitive Reverse Transcriptase Polymerase Chain Reaction Assay, Journal of Clinical Laboratory Analysis, vol. 11, 1997, pp. 258-266.
Mattes et al., Quantitative reverse transcriptase/PCR assay for the measurement of induction in cultured hepatocytes, Chemico-Biological Interactions, vol. 107, Nov. 6, 1997, pp. 47-61.
Helfrich, et al., A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood, British Journal of Cancer, vol. 76, No. 1, Jul. 1997, pp. 29-35.
Seghezzi et al., C. tropicalis CYP52A6 gene encoding cytochrome P450alk3, EMBL Sequence Database, Jun. 27, 1992. Heidelberg DE, AC Z13010.
Seghezzi et al., Cytochrome P450 52
Brenner Alfred A.
Cornett Cathy A.
Craft David L.
Eirich L. Dudley
Eshoo Mark
Achutamurthy P.
Cognis Corporation
Daniels John F.
Pak Yong D.
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