Regulatory region for heterologous gene expression in yeast

Details Regulatory region for heterologous gene expression in yeast Regulatory region for heterologous gene expression in yeast

1985-09-25

1989-08-08

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

536 27, 435 70, 4351723, 435255, 435256, 435320, 935 27, 935 36, 935 37, 935 56, 935 69, C12P 2100, C12P 2102, C12N 1500, C12N 700

Patent

active

048552313

ABSTRACT:
Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.

REFERENCES:
Tuite et al., EMBO, vol. 1, pp. 603-608, 1982, "Regulated High Efficiency Expression of Human Interferon-Alpha in Saccharomyces cerevisiae."
Bennetzen et al., J. Biol. Chem., vol. 257(6), Mar. 25, 1982, pp. 3018-3025, "The Primary Structure of the Saccharomyces cerevisiae-Gene for Alcohol Dehydrogenase 1."
Sibirny et al., Curr. Genet., vol. 8, pp. 107-114, 1984, "Identification of Regulatory Genes of Riboflavin Permeates and .alpha.-glucosidase in the Yeast Pichia guillrermandii".
Stephien et al., Gene, vol. 24 (2-3), 1983, pp. 289-298, "Synthesis of a Human Insulin Gene, 6. Expression of the Synthetic Proinsulin Gene . . . ".

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