Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1986-11-04
1991-06-18
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
43525231, 4353201, 435832, 536 27, 935 8, 935 9, 935 29, 935 41, 935 45, 935 48, 935 61, 935 74, 935 76, C12N 1500, C12N 120
Patent
active
050249436
ABSTRACT:
Novel constructs in plasmids are provided for evaluating the efficiency of expression and secretion of structural genes. The constructs provide for transcriptional and translational regulatory regions, a signal sequence and structural gene, which also may be readily excised and substituted, so as to allow for mixing and matching of regulatory regions, signal sequences and genes to evaluate regions for use in the expression of a desired peptide. Particularly, synthetic regions are provided which may be used with other synthetic regions or wild-type regions.
Plasmids are provided for screening Bacillus genomic sequences for regulatory regions, particularly promoters employing a structural gene secreting an enzyme which can produce a product which allows for visual detection.
REFERENCES:
patent: 4624926 (1986-11-01), Inouye et al.
patent: 4711843 (1987-12-01), Chang
Mongkolsuk et al., 1983, J. Bacteriol 155(3):1399-1406.
Palva et al., 1981, Gene 15:43-51.
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Ortlepp et al., 1983, Gene, 23:267.
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Moran, et al., 1982, MGG, 186:339.
Gryezan et al., 1978, J Bacteriol, 134:318.
Moran et al., 1981, Cell, 25:783.
Gist-Brocades
Peat Richard C.
Rae-Venter Barbara
Rowland Bertram I.
Wiseman Thomas G.
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