Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1981-04-17
1983-09-27
Wiseman, Thomas G.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
435240, 435241, C07G 700, C12N 500, C12N 502
Patent
active
044068301
ABSTRACT:
A cell mediated immune response system glycoprotein having a molecular weight of about 90,000 and having at least one sialic acid moiety as a biologically active site is disclosed. The glycoprotein is specifically bound by wheat germ agglutinin and also by the hydrophobically binding ligant Blue A (Cibacron Blue F3G-A) but does not bind to lysine. The glycoprotein is a necessary cofactor with Interleukin-1 in the biosynthesis of T-cell growth factor (I1-2). A process for producing a serum-free and mitogen-free I1-2 in vitro by adding the glycoprotein to a serum-free- and mitogen-free Interleukin-1 preparation is described. The method for producing the serum-free and mitogen-free Interleukin-1 is also described. A chemically defined T-cell growth culture medium containing the new glycoprotein as the only protein substance is used in the above process and also provides a means for studying regulation of T-cell lymphocyte growth. Inhibitors for the formation of T-cell blasts in cell mediated immunological response provide a powerful new tool for preventing host-graft rejection. These inhibitors are alkylating agents for serine esterase enzymes which have been found to be involved in the cell mediated immunological response system which generates natural Killer (NK) cells, the latter being responsible for graft rejection in a host.
REFERENCES:
patent: 4146603 (1979-03-01), Davidson et al.
Eagle; Science 122, 43 (1955); in Readings in Mammalian Cell Culture, Pollack (ed.), Cold Spring Harbor Laboratory, 1973, pp. 43-46.
Fabricus et al.; "The role of serum proteins in PHA-induced production of interleukin-2 by human peripheral blood lymphocytes," Behring Inst. Mitt. 67, 249-251 (1980).
Fabricius et al., "Human Primary T-Cell Lines in Lectin-Free Media Immunobiol., vol. 156, pp. 364-371 (1979).
Alvarez et al., "Human T Cell Growth Factor;" Dept. of Immunol., pp. 977-983 (1979).
Needleman et al., "Human Lymphocyte Transformation Induced by Mitogens and Antigens in a Serum-free Tissue Culture System", J. of Immunol. Methods, 44 (1981) pp. 3-14.
Fabricius Hans-Ake
Kottgen Eckart U.
Martinell James
Shanksville Corporation N.V.
Wiseman Thomas G.
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