Regulatory gene for the expression of nitrile hydratase gene

Details Regulatory gene for the expression of nitrile hydratase gene Regulatory gene for the expression of nitrile hydratase gene

1995-10-02

1997-11-04

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525411, 435325, 4353201, 536 237, C12N 100, C12N 510, C12N 1531, C12N 1563

Patent

active

056839138

ABSTRACT:
The invention relates to a regulatory gene coding for a polypeptide having the ability to activate a promoter for a nitrile hydratase gene, a recombinant DNA containing said regulatory gene, and a transformant transformed with said recombinant DNA.
The introduction of the regulatory gene of the invention along with a nitrile hydratase gene and its promoter region permits bacteria of the genus Rhodococcus to produce a higher level of nitrile hydratase. Other extraneous gene can also be introduced into a region downstream of the promoter to produce other proteins in high yield.

REFERENCES:
Kobayashi et al. "Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt containing nitrile hydratase genes from Rhodococcus rhodochrous J1." Biochim. Biophys. Acta 1129:23-33, 1991.

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