Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-05-08
2010-10-05
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S091410, C435S006120
Reexamination Certificate
active
07807410
ABSTRACT:
A DNA sequence from the upstream region of cold-inducible hutU gene of the Antarctic Psychrotrophic BacteriumPseudomonas syringae, comprising promoter elements and other regulatory sequences, with unique ‘CAAAA’ nucleotide sequence at −10 site of multiple transcription start sites and using said promoter to express genes of interest in the said bacterium at temperature as low as 4° C. and using the said bacterium with generation time ranging between two and half to three hours, as a system to produce heat labile proteins of pharmaceutical significance.
REFERENCES:
patent: 4824782 (1989-04-01), Furutani et al.
patent: 5728519 (1998-03-01), Levenbook et al.
patent: 5942496 (1999-08-01), Bonadio et al.
patent: 5994076 (1999-11-01), Chenchik et al.
Kannan et al., Histidine utilization operon *hut) is upregulated at low temperature in the Antarctic psyhotrophic bacteriumPseudomonas syringae, FEMS Microbiology Letters 161, 1998, pp. 7-14.
Accession No. AF326719, Ray et al., (2002).
Janiyani Kamala L.
Ray Malay Kumar
Council of Scientific and Industrial Research
Foley & Lardner LLP
Ketter James S
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