Regulation of exoprotein in Staphylococcus aureus

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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530350, 536 231, 536 243, C12Q 168, C07K 1300, C07H 2102, C07H 2104

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055872883

ABSTRACT:
This invention relates to the recognition and control of bacterial infections, particularly infections caused by Staphylococcus aureus (S. aureus). The present invention provides the staphylococcal accessory regulatory (sar) protein, and the gene encoding the sar protein (sar). The present invention also provides a diagnostic method for determining the pathogenicity of an S. aureus isolate using the sar gene.

REFERENCES:
Cheung, et al, "Regulation of Exoprotein Expression by Staphlococcus Aureus by a locus (SAR) distinct from Agr", Jul. 1992, PNAS 89:6462-6466.
Camilli, et al "Insertional Mutagenesis of Listeria monocytogenes with a Novel Tn917 Derivative That Allows Direct Cloning of DNA Flanking Transposon Insertions", Jul. 1990, J. Bacteriology 172:3738-3744.
Cheung et al "Cloning and Sequencing of sarA of Staphylococcus aureus, a Gene required for the Expression of agr", Jul. 1994, J. Bacteriology 176:4168-4172.
McConkey "Human Genetics: The Molecular Revolution" 1993, Jones and Bartlett Publishers, Boston, p. 33.
Sambrook et al, Molecular Cloning-A Laboratory Manual, CSH Press, Cold Spring Harbor, New York, 1989, pp. 17.2-17.44.
Wnendt et al, "Characterization of the gene encoding .alpha.-sarcin, a ribosome-inactivating protein secreted by Aspergillus giganteus" (Mar. 1993), Gene 124:239-244.
Nandivada et al, "SAR-2: Identification of a novel plasmid-encoded .beta..music-flat.lactamase from India", 1989, Fems Microbiol. Lett. 57: 219-222.
Lawton, et al, "Acquired Resistance Signal Transduction in Arabidopsis is Ethylene Independent", 1994, Plant Cell 6:581-588.

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