Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-07-19
2005-07-19
Achutamurthy, Ponnathapura (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S109000, C435S113000, C435S115000, C435S116000, C435S232000, C435S320100, C435S252320, C435S252330, C536S023600, C536S023200
Reexamination Certificate
active
06919190
ABSTRACT:
The present invention provides a method of increasing the productivity of a microorganism by improving the assimilation of carbon dioxide. Specifically, the invention provides a polypeptide having phosphoenolpyruvate carboxylase activity which does not require acetyl coenzyme A for activation and is desensitized to feedback inhibition by aspartic acid, and to genes coding for this polypeptide. A gene encoding a PEP carboxylase that is not regulated by acetyl-CoA or aspartic acid can improve carbon flow from the three carbon intermediate PEP to the four carbon intermediate OAA, contribute to compounds derived from OAA, and increase amino acid biosynthesis. The invention further provides recombinant DNA molecules containing these genes, bacteria transformed with these genes, and a method of producing amino acids using the transformed bacteria.
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Crafton Corey M.
Rayapati P. John
Achutamurthy Ponnathapura
Archer-Daniels-Midland Company
Buchanan & Ingersoll PC
Cochenour Craig G.
Stewart III Duane A.
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