Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1985-03-26
1988-04-12
Tarcza, John E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435205, 435210, 435200, 435 42, 435842, C12N 934, C12N 944, C12N 922, C12P 714
Patent
active
047374594
ABSTRACT:
A method is disclosed for producing catabolite repression-resistant mutant strains of C. thermosulfurogenes and C. thermohydrosulfuricum. The method comprises challenging a wild strain of the organism with nitrosoguanidine, followed by enrichment on 2-deoxyglucose and culturing an iodine stained starch-glucose containing agar. The colonies which convert starch most efficiently are catabolite repression-resistant. Pure cultures of the mutants and methods employing the mutants to prepare enzymes are also described.
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Hyun Hyung-Hwan
Zeikus Joseph G.
Michigan Biotechnology Institute
Tarcza John E.
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