Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-07-12
2005-07-12
Salimi, Ali R. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S007710
Reexamination Certificate
active
06916622
ABSTRACT:
There is provided an amino acid sequence comprised within the PV E1 protein region A delineated by amino acids 352 and 439, and any derivative variant or fragment thereof, necessary for the oligomerization of the E1 protein. This amino acid sequence is capable of self-association and of associating with the full length E1 protein and any derivative, variant or fragment thereof comprising the sequence of this invention. A specific aspect of this first embodiment, the amino acid domain of this invention delimited by amino acids 353 to 438 of the PV E1 protein. More particularly, the amino acid domain of this invention is as defined by SEQ ID NO. 2. There is also provided a cross-linking assay to directly measure the level of oligomerization (or inhibition thereof) of the E1 protein. In accordance with a fourth embodiment of this invention, there is provided a N-terminally truncated E1 protein. More particularly, one aspect of this fourth embodiment encompasses the E1 protein delimited by amino acid 72 to 649 (SEQ ID NO. 78).
REFERENCES:
patent: 5601973 (1997-02-01), Mueller et al.
patent: 5821048 (1998-10-01), Howley et al.
patent: 0 523 395 (1993-01-01), None
patent: WO 96 41018 (1996-12-01), None
patent: WO 99/57283 (1999-11-01), None
Sun, Y. et al: “Active domains of human papillomavirus type 11 E1 protein for origin replication”; Journal of General Virology, vol. 79, Jul. 3, 1998, pp. 1651-1658.
Sedman, J. et al; “the initiator protein E1 binds to the bovine papillomavirus origin of repplication as a trimeric ring. like structure”; EMBO Journal, vol. 15, No. 18, 1996, pp. 5085-5092.
Lusky, M. et al; “The bovine papillomavirus E2 protein modulates the assembly of but is not stably maintained in a replication-competent multimeric E1-replication origin complex”; Proc. Nat'l Acad. Sci. USA, vol. 91, Sep. 1994, pp. 8895-8899.
S-Y Chan, et al; “Phylogenetic analysis of 48 papillomavirus types and 28 subtypes and variants: a showcase oft te molecular evolution of DNA viruses”; Journal of Virology, US, New York, US, vol. 66, No. 10, pp. 5714-5725.
Boehringer Ingelheim (Canada) Ltd.
Devlin Mary-Ellen M.
Morris Michael P.
Pocchiari Susan K.
Salimi Ali R.
LandOfFree
Regions of papilloma virus E1 helicase involved in E1... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Regions of papilloma virus E1 helicase involved in E1..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Regions of papilloma virus E1 helicase involved in E1... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3400483