Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2011-01-04
2011-01-04
Pak, Yong D (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S004000, C435S006120, C435S069100, C435S071100, C435S025000, C435S132000, C435S440000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07863030
ABSTRACT:
Cytochrome P450 BM-3 fromBacillus megateriumwas engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations withE. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.
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Arnold Frances H
Meinhold Peter
Peters Matthew W
Baker, Jr. Joseph R.
Gavrilovich Dodd & Lindsey LLP
Pak Yong D
The California Institute of Technology
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