Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1998-02-02
2000-06-06
Fox, David T.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435420, 435430, 4354301, 435431, 800265, 800268, 8003172, A01H 400, C12N 504, A01C 100
Patent
active
060717467
ABSTRACT:
This invention is directed to a method for producing somatic embryos on plant tissue. Furthermore, somatic embryos may be produced using explants obtained from a broad range of plant species, and using either juvenile or mature tissues. The method involves obtaining a stock tissue culture plantlet by exposing the plant tissue to a medium comprising salts, vitamins and an energy source; preparing an explant from the stock tissue culture plantlet; transferring the explant to a proliferation medium comprising salts, vitamins, an energy source and at least one growth regulator for a period of time sufficient to produce a callused explant; and transferring the callused explant to a medium comprising salts, vitamins, an energy source and at least two growth regulators for a period of time sufficient to produce somatic embryos. Following this method somatic embryos are produce in significantly less time that observed using other somatic embryogenesis protocols. Plants produced from the somatic embryos are easily prepared. Since somatic embryos can be obtained from mature tissues, specific plants of agronomic importance comprising desirable traits, may now be clonally propagated. Furthermore, somatic embryos produced following the method of this invention may be used for the preparation of synthetic seed.
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Douglass L. Katheryn
Seabrook Jane
Agriculture and Agri-Food Canada
Fox David T.
Grunberg Anne Marie
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