Regeneration of chromatography material

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

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Details

C210S670000, C436S824000, C436S828000

Reexamination Certificate

active

06972327

ABSTRACT:
The invention provides improved methods of regenerating and using affinity chromatography material, in particular Protein A affinity chromatography resins.

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patent: 6593097 (2003-07-01), Xu
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Bill et al., “Optimization of protein G chromatography for biopharmaceutical monoclonal antibodies,”J. Mol. Recognit. 8(1/2):90-94, 1995.
Cong et al., “Purification of recombinant human interferon-γ by immunoaffinity chromatography with monoclonal antibody,”Chin. J. Chem. Eng. 3(3):125-133, 1995.
Desamaud et al., “Protein purification using combined streptavidin (or avidin)-Sepharose and thiopropyl-Sepharose affinity chromatography,”J. Chromatography 603(1-2):95-104, 1992.
Fazekas et al, “Reusability of immunoaffinity columns for determination of fumonisins in maize,”Natural Toxins 7(6):259-263, 1999.
Fountoulakis et al., “Reduced binding capacity of concanavalin A-Sepharose after treatment with chaotropic agents,”J. Biochem. Biophys. Methods 27(2):127-132, 1993.
Gagnon,Purification Tools for Monoclonal Antibodies,Validated Biosystems, Inc., Tucson, AZ, 1996, Ch. 9, “Protein A Affinity Chromatography,” pp. 155-198.
Hale et al., “Repeated cleaning of protein A affinity column with sodium hydroxide,”J. Immunol. Methods 171(1):15-21, 1994.
Sidhu, “A novel affinity purification of D-1 dopamine receptors from rat striatum,”J. Biol. Chem. 265(17):10065-10072, 1990.

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