Regeneration of an enzyme immobilizate

Details Regeneration of an enzyme immobilizate Regeneration of an enzyme immobilizate

1979-05-15

1981-06-02

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

435106, 435174, C12N 1114

Patent

active

042712696

ABSTRACT:
An enzyme immobilizate comprising an amorphous siliceous support to which an enzyme is bonded by adsorption via an amino alkoxy silane is regenerated after the enzyme has become inactive by contacting the immobilizate containing the inactive enzyme with a solution of inorganic ammonium sulfate or a solution of primary, secondary, tertiary or quaternary C.sub.1 to C.sub.6 alkyl or hydroxy alkyl ammonium sulfate to desorb the enzyme and then contacting the support with a fresh enzyme solution to adsorb active enzyme to the support.

REFERENCES:
patent: 3519538 (1970-07-01), Messing et al.
Tosz et al., Studies on Continuous Enzyme Reactions, Agr. Biol. Chem., vol. 33, No. 7, 1969, (pp. 1053-1059).
Messing, R. A., Immobilized Enzymes for Industrial Reactors, Academic Press, N.Y., 1975, (pp. 79-95).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 75-82).

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