Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2008-05-06
2008-05-06
Baum, Stuart F. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S278000, C435S410000, C435S469000, C435S430000, C435S431000, C435S420000, C435S468000
Reexamination Certificate
active
11007194
ABSTRACT:
The present invention is directed to a method ofAcacia mangiumregeneration through organogenesis and a method of genetic transformation ofAcacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced toAcacia mangiumviaAgrobacteriuminfection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.
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Hong Yan
Xie Deyu
Baum Stuart F.
Rothwell Figg Ernst & Manbeck P.C.
Temasek Life Sciences Laboratory Limited
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