Regeneration and genetic transformation of Acacia mangium

Details Regeneration and genetic transformation of Acacia mangium Regeneration and genetic transformation of Acacia mangium

2005-01-25

2005-01-25

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Via agrobacterium

C800S278000, C435S468000, C435S469000, C435S410000, C435S430000, C435S431000, C435S420000

Reexamination Certificate

active

06846971

ABSTRACT:
The present invention is directed to a method ofAcacia mangiumregeneration through organogenesis and a method of genetic transformation ofAcacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced toAcacia mangiumviaAgrobacteriuminfection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

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