Regenerable solid phase for carrying out specific binding reacti

Details Regenerable solid phase for carrying out specific binding reacti Regenerable solid phase for carrying out specific binding reacti

1995-10-12

1998-07-21

Spiegel, Carol A.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

435 71, 435970, 436518, 436810, G01N 33553

Patent

active

057834555

ABSTRACT:
The present invention relates to a method for carrying out immunochemical reactions and to regenerable solid phases which can be used for this purpose. When use is made of precious metals, preferably gold, as the solid phase, and also of certain reducing agents or oxidizing agents, such as, for example, sodium borohydride and tetrabutylammonium hydroxide, with or without the addition of detergents, the solid phase can be employed repeatedly, following regeneration.

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Huet et al., "Automatic Apparatus for Heterogeneous Enzyme Immunoassays . . . and Electrochemical Regeneration of the Solid Phase," Analytic Chimica Acta, vol. 272, pp. 205-212, Feb. 12, 1993.
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Phelps et al, "Technology for Regenerable Biosensor Probes Based on Enzyme-Cellulose Binding Domain Conjugates," Biotech. Prog. 10: 430-440 (1994).

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