Regenerable dialkylaminoalkyl cellulose support matrix for immob

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...

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435179, 436524, 436530, 530811, 530814, 536 63, 536 69, 536 84, 536 99, C12N 1114, C12N 1112, G01N 33548, C07K 1712

Patent

active

049332848

ABSTRACT:
A regenerable support matrix useful for immobilization of biologically active materials is prepared by coating a core support with a cellulose ester, removing ester groups by hydrolysis to produce hydroxyl groups and converting the hydroxyl groups to dialkylaminoalkyl ether groups. The support matrix can immobilize biologically active proteinaceous materials with a net negative charge by adsorption. The support matrix is readily regenerated when an immobilized biologically active material becomes inactive by washing the support with a base or salt solution and adsorbing additional biologically active material to the support. Multiple cycles of immobilization and regeneration are possible without significant deleterious affects.

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Kirk-Othmer, "Encyclopedia of Chemical Technology", Third Ed., vol. 9, pp. 155-157.

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