Refractive index detector

Optics: measuring and testing – Refraction testing – Refractive rod engages specimen

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356131, G01N 2146

Patent

active

039998576

ABSTRACT:
A refractive index detector is described comprising a waveguide, means for contacting said waveguide with a fluid, a light source and means to transmit light into said waveguide, means for detecting light exiting from said waveguide as an indication of the refractive index of said fluid and means to automatically change the angle of incidence of the light entering said waveguide in response to changes of refractive index of said fluid. A preferred embodiment of the detector for use as a detector for gradient elution chromatography has means to indicate the rate of change of refractive index. A preferred means to automatically change the angle of incidence of the light entering the waveguide is a movable prism. It is preferred to use a monochromatic light source in the detector. It is preferred to provide reference means for the detector to compensate for changes in intensity of the light output from said light source. A process for determining the refractive index of effluent from a gradient elution liquid chromatograph is described comprising continuously measuring the differential of the refractive index of said effluent to compensate for the change in refractive index resulting from the continuously changing composition of the elution solution.

REFERENCES:
patent: 2569127 (1951-09-01), Eltenton
patent: 2885923 (1959-05-01), Simmons
patent: 3017802 (1962-01-01), Witt
patent: 3370502 (1968-02-01), Wilks
patent: 3520619 (1970-07-01), Ward

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