Reduction of nonspecific hybridization by using novel base-pairi

Details Reduction of nonspecific hybridization by using novel base-pairi Reduction of nonspecific hybridization by using novel base-pairi

1994-08-30

1997-10-28

Elliott, George G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 87, 536 243, 536 2431, 536 2433, 536 263, 536 2672, C12Q 168, C12D 1924, C07H 2102, C07H 1904

Patent

active

056817029

ABSTRACT:
Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.

REFERENCES:
patent: 4868105 (1989-09-01), Urdea et al.
patent: 5124246 (1992-06-01), Urdea et al.
Stratagene Catalog (1988) p. 39.
Chern et al, (1987), "A novel and efficient synthesis of isoguanosine", Tetrahedron Lett. 28(19):2151-2154.
Horn et al, (1995), "Hybridization properties of the 5-methyl-isocytidine/isoguanosine base pair in synthetic oligonucleotides", Tetrahedron Lett. 36(12):2033-2036.
Bain et al, (1992), "Ribosome mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code", Nature 356:537-539.
Switzer et al, (1989), "Enzymatic incorporation of a new base pair into DNA and RNA", J. Am. Chem. Soc. 111:8322-8323.
Voegel et al, (1994), "Nonstandard hydrogen bonding in duplex oligonucleotides. The base pair between an Acceptor-Donor-Donor pyrimidine analog and a Donor-Acceptor-Acceptor purine analog", J. Am. Chem. Soc. 116:6929-6930.
U.S. application No. 08/164,388, Urdea et al., filed Dec. 8, 1993.
Leach et al. "Theoretical Investigations of Novel Nucleic Acid Bases", J. Am. Chem. Soc. (1992) 114:3675-3683.
Tor et al. "Site-Specific Enzymatic Incorporation of an Unnatural Base N.sup.6 -(6-Aminohexyl)isoguanosine, into RNA", J. Am. Chem. Soc. (1993) 115:4461-4467.
Switzer et al. "Enzymatic Recognition of the Base Pair between Isocytidine and Isoguanosine", Biochemistry (1993) 32:10489-10496.
Mantsch et al. "Structural and Enzymatic Properties of Adenine I-Oxide Nucleotides" Biochem. (1993) 14:5593-5601.
Piccirilli et al. "Enzymatic Incorporation of a New Base Pair into DNA and RNA Extends the Genetic Alphabet" Nature (1990) 343:33-37.
Seela et al. "Synthesis of Phosphonates and Oligodeoxybonucleotides Derived from 2'-Deoxyisoguanosine and 2'-Deoxy-2-haloadenosines" Helv. Chim. Acta (1992) 75:2298-2306.
Kazimierczuk et al. "2-Deoxyisoguanosine and Base-Modified Analogues: Chemical and Photochemical Synthesis" Helv. Chim. Acta (1991) 74:1742-1748.
International Search Report mailed Jan. 8, 1996 in the coresponding international application No. PCT/US95/11115.

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