Reduction of background interferences in the molybdate-dye prote

Details Reduction of background interferences in the molybdate-dye prote Reduction of background interferences in the molybdate-dye prote

1993-12-17

1995-03-21

Czaja, Donald E.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

422 56, 436 15, 436 63, 436 87, 436 88, 436169, G01N 3300

Patent

active

053994981

ABSTRACT:
Disclosed is an improvement to the assay for protein in urine involving the use of a molybdate or tungstate salt and an indicator dye which forms a complex with molybdate or tungstate whose absorption band is shifted in the presence of protein. The improvement involves the use of an ionizable phosphate containing compound characterized by the formula: ##STR1## to reduce background interference caused by constituents normally present in urine.

REFERENCES:
patent: 4960710 (1990-10-01), Lau
patent: 5055407 (1991-10-01), Lau et al.
patent: 5087575 (1992-02-01), Lau
patent: 5173431 (1992-12-01), Pugia et al.

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