Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-04
1998-01-06
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 75, 435 794, 436518, C12Q 168, G01N 3353, G01N 33547, G01N 33543
Patent
active
057053383
ABSTRACT:
Novel non-competitive assay techniques have been developed which not only improve sensitivity, but also are convenient and less susceptible to interfering factors. They are compatible with existing instruments and can be run in one or more test tubes. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured. Variations of the procedure include the use of a reversible bridge for attaching the insoluble material to the analyte mimic and the conduct of the assay in various porous media, such as paper, chromatographic and electrophoretic media, and dipsticks.
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Livshin Laurie Ann
Martinelli Richard A.
Piran Uri
Riordan William J.
Unger John T.
Blackburn Robert P.
Chiron Diagnostics Corporation
Knode Marian C.
Morgenstern Arthur S.
Wortman Donna C.
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