Reduction in non-specific interference in hydrophobic ligand ass

Details Reduction in non-specific interference in hydrophobic ligand ass Reduction in non-specific interference in hydrophobic ligand ass

1983-06-02

1985-05-21

Nucker, Christine M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

436529, 436800, 436825, G01N 3352

Patent

active

045187010

ABSTRACT:
Methods are provided for reducing non-specific interference in competitive protein binding assays employing as the labeled reagent a fluorescent conjugate of a hydrophobic ligand conjugated to a fluorescer, which in turn is bound to a water soluble polysaccharide carrier ("fluorescer conjugate reagent"). In order to reduce non-specific interference from physiologic samples, the fluorescent reagent is combined with a lipid substituted neutral support, under conditions which provides two binding fractions: a first weakly binding fraction which is relatively free of non-specific interference in a competitive protein binding assay employing physiological fluids; and a second fraction, which more strongly binds to the lipid substituted support.

REFERENCES:
Smith et al., Chem. Abstracts, 89, (1978), #74057.
Lisi et al., Clinica Chimica Acta, 120, (1982), pp. 171-179.

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