Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2011-04-05
2011-04-05
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
Reexamination Certificate
active
07919268
ABSTRACT:
Disclosed are methods and kits for measuring enzyme activity in which the turbidity of a sample caused by cell components is minimized by particular additives. The methods include the steps of providing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction. In particular, a method is disclosed wherein the enzyme is lactate dehydrogenase (LDH) and the first additional substance to stop the reaction is HCl.
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Nikolaus Thomas
Rode Hans-Juergen
Watzele Manfred
Gitomer Ralph
Roche Diagnostics Operations Inc.
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