Reducing interference in ligand-receptor binding assays

Details Reducing interference in ligand-receptor binding assays Reducing interference in ligand-receptor binding assays

1985-05-09

1988-04-12

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435810, 436518, 436537, 436547, 436548, 436808, 436817, 436818, 436819, 436825, 935108, 935110, G01N 3353

Patent

active

047374560

ABSTRACT:
A method for assaying for a ligand analyte which is a member of a specific binding pair ("sbp member") consisting of ligand and its complementary receptor is disclosed. The ligand analyte has a binding site in common with an interfering substance present in a sample suspected of containing the analyte. The interfering substance has at least two binding sites. The method comprises combining in an assay medium without intervening separation (1) the sample, (2) a blocking receptor which does not bind to the ligand and does bind to the interfering substance, thereby blocking the binding of a common receptor to the interfering substance, and (3) a common receptor which binds to the common binding site. Any additional members of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample are added to the assay medium. Next, the assay medium is examined for the presence of a detectable signal. The method has application to both heterogeneous and homogeneous assays.

REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein
patent: 4376110 (1983-03-01), David
Wada Clinical Chemistry 28(9), pp. 1862-1866 (1982).

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