Reducing branching and enhancing fragmentation in culturing...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S006120, C435S041000, C435S069100, C435S183000, C435S252300, C435S254100, C435S325000, C536S023200, C536S023400, C536S023700, C536S023740

Reexamination Certificate

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07022526

ABSTRACT:
The invention relates to the field of microorganisms and to the culturing of microorganisms. Means and methods are described for enhancing the culturing properties of filamentous microorganisms, particularly filamentous fungi. According to the invention, the means and methods generally comprise reducing the branching and/or enhancing the fragmentation of the microorganisms, so that their liquid culturing properties are improved. In one embodiment, this is achieved by providing the microorganisms with activity capable of enhancing fragmentation and/or reducing branching such as the activity which in, for example,Streptomyces griseusis encoded by ssgA.

REFERENCES:
Kwak et al., Differential Regulation of ftsZ Transcription during Septation ofStreptomyces griseus, Journal of Bacteriology, Sep. 2001, pp. 5092-5101, vol. 183, No. 17.
Kawamoto, Shinichi, et al., “Cloning and Characterization of a Gene Involved in Regulation of Sporulation and Cell Division ofStreptomyces griseus,”Actinomycetol., vol. 9, No. 2, 1995, pp. 136-151.
Kawamoto, Shinichi, et al., “Expression Analysis of the ssgA Gene Product, Associated with Sporulation and Cell Division inStreptomyces griseus,”Microbiology, vol. 143, 1997, pp. 1077-1086.

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